CRISPR/Cas9: new tools for genome editing

Venue : Room A105, Polo Ferrari 1

At: 14.30

• Prof. Anna Cereseto

Abstract:

Since early days of DNA discovery, the biggest frontier for molecular biologists has been represented by genetic engineering of live cells, genome editing. Initial results in targeted gene modification through natural meganucleases gave rise to new paths forward in the field of genome editing anticipating the development of more specific and flexible artificial nucleases such as zinc finger nucleases (ZFNs) and transcription activator-like effector nuclease (TALEN). The turning point in genome editing occurred with the discovery of CRISPR/Cas9. Theses nucleases of bacterial origin, offer to basic scientists as well to applied biomedical investigators revolutionary tools to modify genomes in live cells. Now, the landmark demonstration that delivery of the CRISPR/Cas9 system can easily correct genetic defects provided direct evi¬dence of how this technology may be used to improve human health. Nevertheless, several crucial challenges remain. The next frontiers in genome editing mediated by CRISPR/Cas9 will be presented.