From single molecules to intracellular sensing for future diagnostic applications

July 22nd 2016
Versione stampabile

Venue: Edificio Povo 2, via Sommarive nr. 9, Povo (Tn) - Room B103
 At 2:00 p.m.

  • Angel Orte - Department of Physical Chemistry, Faculty of Pharmacy Campus Cartuja, UGR University of Granada, Spain

In this talk, I will review our recent developments in understanding biomedical-relevant interactions, down at the single molecule level. Advanced multicolor single-molecule fluorescence techniques have provided tools to unravel complex energy landscapes of protein – protein interactions. For instance, single-molecule experiments have shed some light into the recognition and binding of active partners to ubiquitin chains, and the mechanism of aberrant aggregation of natural proteins into amyloid fibrils, linked to high-impact diseases such as Alzheimer's or Parkinson's. Moreover, the outstanding sensitivity of fluorescence microscopy has motivated us towards the development of novel dye- and nanoparticle-based sensing strategies. I will also review our latest approaches for intracellular, real-time sensing, aimed for osteoporosis and cancer diagnostics.

Seminar held in the framework of the EU project miRNA-DisEasy funded within the programme H2020-MSCA-RISE (GA n° 690866)