Membrane shaping by the BAR domain superfamily proteins and the extracellular vesicles by the shedding of filopodia

External Seminar
30 January 2023
Start time 
3:30 pm
Polo Ferrari 2 - Via Sommarive 9, Povo (Trento)
Room B109
Department of Cellular, Computational and Integrative Biology - CIBIO
Target audience: 
University community
Contact person: 
Department of Cellular, Computational and Integrative Biology - CIBIO
Contact details:


  • Shiro Suetsugu: Nara Institute of Science and Technology, Japan 


There are various membrane curvatures of the cellular membrane. The Bin-Amphiphysin-Rvs (BAR) family domain proteins are the scaffolds for membrane curvatures. Typically, BAR domain proteins are involved in endocytic pit formation and vesicle formation. In contrast, the I-BAR domain proteins, including Missing-in-metastasis (MIM) and IRSp53, promote filopodia formation. However, the involvement of the BAR domain proteins in extracellular vehicles (EVs) generation remains unclear. We found that the filopodia that are induced by the I-BAR domain proteins are susceptible to being shredded into EVs. Interestingly, the shedding was promoted by in vivo equivalent external forces. These results indicated that some populations of EVs are generated from I-BAR-domain-dependent filopodia.