Conference / Meeting

Exploiting Targeted Epigenome Editing for Therapeutic Applications

Angelo Lombardo - San Raffaele Telethon Institute for Gene Therapy and Vita-Salute San Raffaele University, Milan
16 May 2024
Start time 
10:30 am
Polo Ferrari 1 - Via Sommarive 5, Povo (Trento)
Room A101
Department of Cellular, Computational and Integrative Biology - CIBIO
Target audience: 
University community
Contact person: 
Department of Cellular, Computational and Integrative Biology - CIBIO
Contact details:

Epigenome editing is emerging as a powerful new strategy to silence gene expression without altering its primary DNA sequence. In this regard, we and others have previously shown that transient delivery of Engineered Transcriptional Repressors (ETRs) can lead to efficient, long-term stable and specific epigenetic silencing of endogenous genes in both human and mouse cell lines.

The ETRs are chimeric proteins composed of a programmable DNA binding domain, such as CRISPR-Cas9 or ZFPs, fused to either one or more of the following epigenetic repressive domains: KRAB, the catalytic domain of DNMT3A, and DNMT3L. Whether the ETR technology could program efficient and long-lasting gene silencing in clinically relevant cell types and in vivo remains unknown. During my talk, I will present our efforts to improve and characterize the technology towards its in vivo application and further describe application of the platform for cancer immunotherapy.

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