Establishing the activity landscape of the oncogenic kinase Rio1.

Venue: Edificio Povo 2, via Sommarive nr. 9, Povo (Tn) - Room B105
 at 2:00 p.m.

• Peter De Wulf -  Department of Experimental Oncology, European Institute of Oncology, Milan, Italy

The essential kinase Rio1 promotes the maturation of the small ribosome subunit. Other roles are unknown for any eukaryote. Using the budding yeast Saccharomyces cerevisiae we have probed Rio1 activity at the whole-cell level and find that it spatiotemporally integrates chromosome segregation, ribosome biogenesis and activity, chromatin remodelling and gene expression, cell metabolism and physiology to promote growth and viability. Rio1 acts at the protein, DNA and RNA levels. The Rio1 activity network indicates that a pathological expression of the kinase, as observed in myriad cancers, will obstruct the execution of central metabolic pathways and deregulate key growth and division processes, anomalies that are closely associated with tumorigenesis.