The ATPases RUVBL1 and RUVBL2 as regulators of cell cycle and mitosis

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Venue: Edificio Povo 2, via Sommarive nr. 9, Povo (Tn) - Room B101
 At 2:00 p.m.

  • Stefano Ferrari -  Institute of Molecular Cancer Research University of Zurich, Zurich, Switzerland

RUVBL1 and RUVBL2 are integral components of multisubunit protein complexes involved in processes ranging from cellular metabolism, transcription and chromatin remodeling to DNA repair. We have obtained evidence that the heterodimeric complex formed by the two proteins splits in cytokinesis, with RUVBL1 co-localizing and being phosphorylated by Polo-like kinase-1 (PLK1). RNA interference studies showed that depletion of RuvB-like proteins causes severe defects in chromosome alignment and segregation of sister chromatids. Finally, we demonstrate that the ATPase activity of RUVBL1 appears to be indispensable for cell proliferation.