Seminario

Membrane shaping by the BAR domain superfamily proteins and the extracellular vesicles by the shedding of filopodia

Seminario esterno
30 gennaio 2023
Orario di inizio 
15:30
Polo Ferrari 2 - Via Sommarive 9, Povo (Trento)
Aula B109
Organizzato da: 
Dipartimento di Biologia Cellulare, Computazionale e Integrata - CIBIO
Destinatari: 
Comunità universitaria
Partecipazione: 
Ingresso libero
Referente: 
Dipartimento di Biologia Cellulare, Computazionale e Integrata - CIBIO
Contatti: 
comunicazione.cibio@unitn.it

Speaker

  • Shiro Suetsugu: Nara Institute of Science and Technology, Japan 

Abstract

There are various membrane curvatures of the cellular membrane. The Bin-Amphiphysin-Rvs (BAR) family domain proteins are the scaffolds for membrane curvatures. Typically, BAR domain proteins are involved in endocytic pit formation and vesicle formation. In contrast, the I-BAR domain proteins, including Missing-in-metastasis (MIM) and IRSp53, promote filopodia formation. However, the involvement of the BAR domain proteins in extracellular vehicles (EVs) generation remains unclear. We found that the filopodia that are induced by the I-BAR domain proteins are susceptible to being shredded into EVs. Interestingly, the shedding was promoted by in vivo equivalent external forces. These results indicated that some populations of EVs are generated from I-BAR-domain-dependent filopodia.